Various whole cell lysates were separated by 4 - 20% SDS - PAGE, and the membrane was blotted with anti- TSG101 antibody. The HRP - conjugated Goat anti - Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: K562 Lane 3: NIH - 3T3 Lane 4: PC - 12 Predicted band si ze: 44kDa Observed band size: 44kDa

Various whole cell lysates were separated by 4 - 20% SDS - PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800- conjugated Goat anti - Rabbit antibody was used to detect the antibody. Lane1: HL60 - Human promyelocytic leukem ia cell Lane2: HUVEC - Human umbilical vein endothelial cell Lane3: Human CAF -Human cancer- associated fibroblast Lane4: HCT116 - Human colorectal carcinoma Lane5: A549 - Human lung carcinoma Lane6: Jurkat - Human T lymphocyte leukemia Lane7: HT22 - Mouse hippocampal neuronal Lane8: Thp - 1 - Human monocytic leukemia Lane9: HK- 2 - Human pro ximal tubular epithelial Lane10: PC - 12 - Rat adrenal pheochromocytoma Lane11: 3T3 - L1 -Mouse embryonic fibroblast Lane12: PC - 3 - Human prostate adenocarcinoma Lane13: U - 14 - Mouse cervical carcinoma Lane14: 293F - HEK293 derivative, adapted for suspension culture Predicted band size: 44kDa Observed band size: 44kDa