His-Tag Mouse Monoclonal Antibody

Catalog NO.：BE2019
Applications ：WB, IP, IF
Reactivity ：N/A

货号	规格	品牌	库存	价格	数量	操作
BE2019-10	10μl/支
BE2019-100	100μl/支

产品详情
参考文献

Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. His-tag mouse mAb recognizes His-tag placed at N-terminal, C-terminal, and internal regions of fusion proteins.

Product Name:	His-Tag Mouse Monoclonal Antibody
Isotype:	IgG1
Storage Buffer :	1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage instructions:	His antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Recommended dilutions:	WB 1:5000-2000 IP 1:200 IF 1:1000
Optimal dilutions should be determined by the end user.
Specificity：
Alternative Names：
Form:	Liquid
Reactivity:	N/A

A: HEK 293 cell lysate without transfected B: HEK 293 cell lysate transfected with his-tag protein C: IP (PBS + anti-HIS mAb 4℃ overnight) D: IP (HEK293 transfected+protein G+anti-HIS mAb 4℃ overnight)) E: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) F: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain

IF analysis of 293T cells transfected with a HIS-tagged protein, using EASYbio HIS-Tag (6E8) Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-HIS)

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