V5-Tag Mouse Monoclonal Antibody

Catalog NO.：BE2032
Applications ：WB, IP, IF
Reactivity ：N/A

货号	规格	品牌	库存	价格	数量	操作
BE2032-10	10μl/支
BE2032-100	100μl/支

产品详情
参考文献

V5 tag Mouse mAb is the excellent antibody in the research. V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. V5 Tag Antibody can recognize C-terminal, internal, and N-terminal V5-tagged proteins.

Product Name:	V5-Tag Mouse Monoclonal Antibody
Isotype:	IgG1
Storage Buffer :	1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage instructions:	V5 antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Recommended dilutions:	WB 1:2000-5000 IP 1:200 IF 1:1000
Optimal dilutions should be determined by the end user.
Specificity：
Alternative Names：
Form:	Liquid
Reactivity:	N/A

A: HEK 293 cell lysate transfected without V5-tag protein B: HEK 293 cell lysate transfected with V5 -tag protein C: IP (HEK 293 cell without transfected + anti- V5 mAb 4℃ overnight) D: IP (PBS + anti- V5 mAb 4℃ overnight) E: IP (HEK293 transfected+protein G+anti- V5 mAb 4℃ overnight) F: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) G: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain

IF analysis of 293T cells transfected with a V5-tagged protein, using EASYBIO V5-Tag (4C9) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-V5)

[1]Zhao, W., Lu, H., Zhu, J., Luo, L., & Cui, F. (2025). A double-agent microRNA regulates viral cross-kingdom infection in animals and plants. The EMBO journal, 10.1038/s44318-025-00405-4. Advance online publication. https://doi.org/10.1038/s44318-025-00405-4